Classification Glycoside hydrolase

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glycoside hydrolases classified ec 3.2.1 enzymes catalyzing hydrolysis of o- or s-glycosides. glycoside hydrolases can classified according stereochemical outcome of hydrolysis reaction: can classified either retaining or inverting enzymes. glycoside hydrolases can classified exo or endo acting, dependent upon whether act @ (usually non-reducing) end or in middle, respectively, of oligo/polysaccharide chain. glycoside hydrolases may classified sequence or structure based methods.


sequence-based classification

sequence-based classifications among powerful predictive method suggesting function newly sequenced enzymes function has not been biochemically demonstrated. classification system glycosyl hydrolases, based on sequence similarity, has led definition of more 100 different families. classification available on cazy (carbohydrate-active enzymes) web site. database provides series of regularly updated sequence based classification allow reliable prediction of mechanism (retaining/inverting), active site residues , possible substrates. online database supported cazypedia, online encyclopedia of carbohydrate active enzymes. based on three-dimensional structural similarities, sequence-based families have been classified clans of related structure. recent progress in glycosidase sequence analysis , 3d structure comparison has allowed proposal of extended hierarchical classification of glycoside hydrolases.








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